2022
International Conference and Expo on Toxicology and Applied Pharmacology 2022
June 14 and 15, 2022
Cytotoxicity effect of functionalized electrospun nanofibers
Biocompatibility and cytotoxicity studies are one of the most important features that have to be analyzed in samples that are proposed as biomedical devices. Electrospun nanofibers have been gaining much attention in the biomedical industry, because they are interesting tridimensional structures that resemble extracellular matrix in tissue and can be fabricated with a great variety of polymers that confers to the fibrous scaffolds certain interesting characteristics such as biocompatibility, biodegradability, high surface area, bioactivity, adequate mechanical properties, amongst others. These electrospun fibers can be proposed in drug delivery systems, tissue engineering, biotechnology, biosensors, and other areas where cytotoxicity is the most important characteristic. In our group, cytotoxicity has been tested through the MTT assay which tests the mitochondrial activity of viable cells (fibroblasts and polymorphonuclear leukocytes), several polymeric nanofibers such as poly (caprolactone), poly (vinyl alcohol), and poly (vinyl pyrrolidone) functionalized with bioactive molecules such as Ruthenium complex, graphene, silver nanoparticles, curcumin and some pharmaceutical drugs such as dexamethasone phosphate, sildenafil citrate, and propranolol. Moreover, some in vivo analyses have been used to test irritability and toxicity in the tissue of animal models following the ISO-10993-1. Also, bioactive antibacterial activity has been tested using Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Most of the electrospun fibrous scaffolds demonstrated biocompatibility and slight effect in all the cells tested and in vivo animals provide evidence of the potential of the samples to be used in biomedical applications. This work discusses cell response, viability, and characteristics of the nanofibers made by the electrospinning technique, the parameter used, and drawbacks.
Biocompatibility and cytotoxicity studies are one of the most important features that have to be analyzed in samples that are proposed as biomedical devices. Electrospun nanofibers have been gaining much attention in the biomedical industry, because they are interesting tridimensional structures that resemble extracellular matrix in tissue and can be fabricated with a great variety of polymers that confers to the fibrous scaffolds certain interesting characteristics such as biocompatibility, biodegradability, high surface area, bioactivity, adequate mechanical properties, amongst others. These electrospun fibers can be proposed in drug delivery systems, tissue engineering, biotechnology, biosensors, and other areas where cytotoxicity is the most important characteristic. In our group, cytotoxicity has been tested through the MTT assay which tests the mitochondrial activity of viable cells (fibroblasts and polymorphonuclear leukocytes), several polymeric nanofibers such as poly (caprolactone), poly (vinyl alcohol), and poly (vinyl pyrrolidone) functionalized with bioactive molecules such as Ruthenium complex, graphene, silver nanoparticles, curcumin and some pharmaceutical drugs such as dexamethasone phosphate, sildenafil citrate, and propranolol. Moreover, some in vivo analyses have been used to test irritability and toxicity in the tissue of animal models following the ISO-10993-1. Also, bioactive antibacterial activity has been tested using Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Most of the electrospun fibrous scaffolds demonstrated biocompatibility and slight effect in all the cells tested and in vivo animals provide evidence of the potential of the samples to be used in biomedical applications. This work discusses cell response, viability, and characteristics of the nanofibers made by the electrospinning technique, the parameter used, and drawbacks.
Pharma 2022
Incorporation of silver nanoparticles in electrospun nanofibers and their effect on the antimicrobial activity
Anayanci Mendoza Villicana1, Yadira Gochi Ponce1, Daniel Grande2, José Manuel Cornejo Bravo3, Arturo Zizumbo López1, Marlon César González Joaquín1, Alejandra Rocio Chávez Santoscoy4, Juan Antonio Paz González5, Nina Bogdanchikova6, Graciela Lizeth Pérez González3,5, Luis Jesús Villarreal-Gómez3,5
1Tecnológico Nacional de México/Instituto Tecnológico de Tijuana, Blvd. Alberto Limón Padilla y Av. ITR Tijuana S/N, Colonia Mesa de Otay C.P. 22500 Tijuana, Baja California, México, Tijuana, Baja California, México
2Institut de Chimie et des Matériaux Paris-Est, ICMPE – CNRS, Thiais, Paris, France, Thiais, Paris, Francia
3Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Unidad Otay, Tijuana, Baja California, México, Tijuana, Baja California, México
4Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, C.P. 64849. Monterrey, Nuevo Léon, México
5Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Unidad Valle de las Palmas, Tijuana, Baja California, México, Tijuana, Baja California, México
6Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, México, Ensenada, Baja California, México
Abstract
Wound dressings technology is of major interest in the actuality in order to avoid undesired effects when burns or skin wounds are treated, especially their risk for microbial contamination and subsequent infection. Hence, silver nanoparticles had claimed importance as antimicrobial agents and their use has been increased in the biomedical field. Hence, a comparative study of different methods to synthesize electrospun polymeric fibers loaded with silver nanoparticles (PCL / PVP-AgNPs) was investigated and performed such as direct blending, ultraviolet radiation, thermal treatment, and silver mirror reaction methods, and its antimicrobial efficiency is reported as well. The morphology, structure, and size of the fibers were obtained using the scanning electron microscope (SEM) where the loading of the AgNPs in the fibers and their distribution was demonstrated. Fourier transform infrared spectroscopy and Raman spectroscopy was used to measure the physicochemical properties of the fibers. The dynamic light scattering study made it possible to measure the size of the nanoparticles and the surface charge of the samples. The antimicrobial study was carried out for 24, 48, and 72 h in Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, and cytotoxicity was evaluated with the MTT assay using HFF-1 human fibroblast cells. The results showed that the method with the best antimicrobial effect in both bacteria is the one where a reduction by ultraviolet light was performed to load the AgNPs. In SEM micrographs, the best distribution of AgNPs on the fiber was observed in the silver mirror reaction method, but UV radiation promote this distribution efficiency, the physicochemical properties obtained were desirable, and the particle size falls within the range to be considered Ag nanoparticle (~17 nm). These results determined the best method to prepare antibacterial wound dressings.
Keywords: Silver nanoparticles; Nanofibers; Electrospinning; UV; Thermal treatment; silver mirror reaction; direct blending
Anayanci Mendoza Villicana1, Yadira Gochi Ponce1, Daniel Grande2, José Manuel Cornejo Bravo3, Arturo Zizumbo López1, Marlon César González Joaquín1, Alejandra Rocio Chávez Santoscoy4, Juan Antonio Paz González5, Nina Bogdanchikova6, Graciela Lizeth Pérez González3,5, Luis Jesús Villarreal-Gómez3,5
1Tecnológico Nacional de México/Instituto Tecnológico de Tijuana, Blvd. Alberto Limón Padilla y Av. ITR Tijuana S/N, Colonia Mesa de Otay C.P. 22500 Tijuana, Baja California, México, Tijuana, Baja California, México
2Institut de Chimie et des Matériaux Paris-Est, ICMPE – CNRS, Thiais, Paris, France, Thiais, Paris, Francia
3Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Unidad Otay, Tijuana, Baja California, México, Tijuana, Baja California, México
4Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, C.P. 64849. Monterrey, Nuevo Léon, México
5Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Unidad Valle de las Palmas, Tijuana, Baja California, México, Tijuana, Baja California, México
6Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, México, Ensenada, Baja California, México
Abstract
Wound dressings technology is of major interest in the actuality in order to avoid undesired effects when burns or skin wounds are treated, especially their risk for microbial contamination and subsequent infection. Hence, silver nanoparticles had claimed importance as antimicrobial agents and their use has been increased in the biomedical field. Hence, a comparative study of different methods to synthesize electrospun polymeric fibers loaded with silver nanoparticles (PCL / PVP-AgNPs) was investigated and performed such as direct blending, ultraviolet radiation, thermal treatment, and silver mirror reaction methods, and its antimicrobial efficiency is reported as well. The morphology, structure, and size of the fibers were obtained using the scanning electron microscope (SEM) where the loading of the AgNPs in the fibers and their distribution was demonstrated. Fourier transform infrared spectroscopy and Raman spectroscopy was used to measure the physicochemical properties of the fibers. The dynamic light scattering study made it possible to measure the size of the nanoparticles and the surface charge of the samples. The antimicrobial study was carried out for 24, 48, and 72 h in Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, and cytotoxicity was evaluated with the MTT assay using HFF-1 human fibroblast cells. The results showed that the method with the best antimicrobial effect in both bacteria is the one where a reduction by ultraviolet light was performed to load the AgNPs. In SEM micrographs, the best distribution of AgNPs on the fiber was observed in the silver mirror reaction method, but UV radiation promote this distribution efficiency, the physicochemical properties obtained were desirable, and the particle size falls within the range to be considered Ag nanoparticle (~17 nm). These results determined the best method to prepare antibacterial wound dressings.
Keywords: Silver nanoparticles; Nanofibers; Electrospinning; UV; Thermal treatment; silver mirror reaction; direct blending
2019
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5to Congreso Internacional de Investigación Tijuana (CI2T), 3 al 5 de abril del 2019, Tijuana, Baja California, México
2018
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2nd Edition of Global Conference on Pharmaceutics and Drug Delivery Systems 2018
Magnus Group takes a great pride in declaring the “2nd Edition of Global Conference on Pharmaceutics and Drug Delivery Systems” (PDDS 2018) which was held in Rome, Italy, during June 04-06, 2018.
Magnus Group takes a great pride in declaring the “2nd Edition of Global Conference on Pharmaceutics and Drug Delivery Systems” (PDDS 2018) which was held in Rome, Italy, during June 04-06, 2018.
Conference Proceedings
Erick Torres, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Juvenal Pantoja, Aracely Serrano-Medina, Ana leticia Iglesias, Graciela Lizeth Pérez González, Luis Jesús Villarreal-Gómez: Microfibras Cargadas con Citrato de Sildenafil como Sistema de Liberación de Fármaco para el Posible Tratamiento de Hipertensión Arterial Pulmonar Crónica. 33° Congreso Latinoamericano de Química, CLAQ y el X Congreso de Ciencias Químicas, Tecnología e Innovación, QUIMICUBA’2018, La Habana, Cuba; 10/2018
Erick Torres, Lupita Elizabeth Martínez Topete, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Juvenal Pantoja, Aracely Serrano-Medina, Ana leticia Iglesias, Graciela Lizeth Pérez González, Luis Jesús Villarreal-Gómez, Tanya Bustamante Reynoso: Fabricación de Membranas Poliméricas Cargadas con Citrato de Sildenafil como Sistema de Liberación de Fármaco para el posible tratamiento de Hipertensión Arterial Pulmonar Crónica. VII Congreso Nacional de la Sociedad Mexicana de Ciencia y Tecnología de Membranas, A.C., Guanajuato, Guanajuato, México; 06/2018
Luis Jesús Villarreal-Gómez, Erick Torres, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Juvenal Pantoja, Aracely Serrano-Medina, Ana leticia Iglesias, Graciela Lizeth Pérez González: Poly (Vinyl Pyrrolidone) Nanofibers as Drug Delivery of Sildenafil Citrate. 2nd Edition of Global Conference on Pharmaceutics and Drug Delivery Systems, Roma, Italia; 06/2018
Erick Torres, Graciela Lizeth Pérez González, Aracely Serrano Medina, Daniel Grande, Ricardo Vera-Graziano, José Manuel Cornejo-Bravo, Luis Jesús Villarreal-Gómez: Electrospun Microfibers Containing Sildenafil Citrate for Fast Dissolving Delivery, for Possible Treatment of Chronic Pulmonary Hypertension. IV Symposium of Nanosciences and Nanomaterials, Ensenada, Baja California, México; 04/2018
Daniella Alejandra Pompa-Monroy, Ricardo Vera-Graziano, Syed Dastager, Karla Juarez-Moreno, N. Bogdanchikova, Irma Esthela Soria Mercado, Ana leticia Iglesias, Meghana Thorat, Luis Jesús Villarreal-Gómez: Gelatin/Collagen Scaffolds and Their Potential Use as a Bacterial Growth Promoter. IV Symposium of Nanoscience and Nanomaterials, Ensenada, Baja California, México; 04/2018
Paola Rachel Rodríguez Medrano, Karen Beldai Robles Campillo, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Hermelinda De la Cruz Durán, Ana leticia Iglesias, Aracely Serrano-Medina, Luis Jesús Villarreal-Gómez: Nanofibras de Poli (vinil pirrolidona) /Propanolol como Potencial Tratamiento en Hemangioma Infantil. 1er Congreso Internacional de Ciencias de la Ingeniería y Tecnología (ECITEC 2017), Tijuana, Baja California, México; 09/2017
Erick Torres, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, José Manuel Cervantes-Uc, López Maldonado Eduardo Alberto, Ana leticia Iglesias, Aracely Serrano Medina, Luis Jesús Villarreal-Gómez, Tanya Bustamante Reynoso: Estudio de Nanofibras de Poli (Vinil Pirrolidona) y Poli (Vinil Alcohol) Cargados con Sildenafil como Sistemas de Liberación de Fármaco. 1er Congreso Internacional de Ciencias de la Ingeniería y Tecnología (ECITEC 2017), Tijuana, Baja California, México; 09/2017
Paola Rachel Rodríguez Medrano, Luis Jesús Villarreal-Gómez, Karen Beldai Robles Campillo, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Karla Oyuki Juárez Moreno, Hermelinda De la Cruz-Durán, Ana leticia Iglesias, Aracely Serrano-Medina: Poly (Vinyl Pirrolidone)/Propanolol Nanofibers as a Potential Treatment of Child Hemangioma. International Conference on Polymers and Advanced Materials POLYMAT-2017, Huatulco, Oaxaca, México; 09/2017
Alan Alvarez, Rodolfo Velasco, Vareska Lucero Zárate-Córdoba, Luis Jesús Villarreal-Gómez, Ana leticia Iglesias, E.A. López-Maldonado, Mercedes Oropeza: Producing Porous Scaffolds for Tissue Engineering: A comparative Analysis between Three Different Methods. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.20775.75689
Rodolfo Velasco, Alan Alvarez, Luis Jesús Villarreal-Gómez, Ana leticia Iglesias, E.A. López-Maldonado, Mercedes Oropeza: Evaluating the Bacterial Adsorption on Electrospun Nanofibers. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.21614.61762
Dayana Michelle Gómez Quintana, Luis Jesús Villarreal-Gómez, Paz Gon-zález Juan Antonio, López Maldonado Eduardo Alberto, Mercedes Oropeza, Cervantes Uc José Manuel, Ricardo Vera-Graziano, Ana leticia Iglesias: Fabricación de Nanofibras de PLGA con Recubrimiento de Polianilina para Aplicación en Ingeniería de Tisular. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.28325.50407
Juliane Jacqueline Coronel-Hurtado, Luis Jesús Villarreal-Gómez, Juan Antonio Paz-González, E.A. López-Maldonado, Teresita Merce-des Oropeza-Guzmán, José Manuel Cervantes-Uc, Ricardo Vera-Graziano, Ana leticia Iglesias: Fabrication of a Composite Material System as Potential Prosthetic Material. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.14903.73126
Vareska Lucero Zárate-Córdova, Luis Jesús Villarreal-Gómez, E.A. López-Maldonado, María Teresita Oropeza-Guzmán, Juan Antonio Paz-González, Ana leticia Iglesias: A Methodology for Fabrication of Hydroxyapatite and Fluoropatite Porous Scaffolds by Salt Leaching Technique for Bone Tissue Regeneration. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.35036.39043
Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Jose Trinidad Lopez Maldonado, Luis Jesús Villarreal Gómez: Biopolímeros naturales y modificados con aplicaciones en medio ambiente, salud y agricultura sustentable. Congreso de Investigación Academia de Journals Tabasco 2016, Villahermosa, Tabasco; 03/2016
Vareska Zarate Cordova, Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Benjamin Gonzalez Vizcarra, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez: SYNTHESIS OF BONE-REPLACEMENT MATERIAL BY SALT-LEACHING METHOD FOR IMPROVE BONE TISSUE REGENERATION. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.2512.5525
Rodolfo Daniel Velasco Barraza, Alan Saul Alvarez Suarez, Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Adriana Alvarez Andrade, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez: Fabricación y caracterización de nanofibras de diferentes polímeros por electrohilado como soporte de sustrato para la construcción de un futuro biosensor. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.4609.7047
Antonio Juarez Duran, Ana Leticia Iglesias, Valentin Miranda soto, Luis Jesús Villarreal Gómez: Síntesis de ligantes del tipo Bis(imino) bi-dentados y tri-dentados con anilina pentafluorada y 3-amino, 5-metil pirazol. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.1988.2649
Alan Saul alvarez Suarez, Eduardo alberto Lopez Maldonado, Maria Teresita Oropeza Guzman, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez, Rodolfo Daniel Velasco Barraza: SÍNTESIS Y CARACTERIZACIÓN DE MATERIALES COMPUESTOS BIOABSORBIBLES PARA APLICACIONES OSEAS. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.4085.4169
Sasha Lizbeth Gomez Castellanos, Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Ana Leticia Iglesias, Eduardo Serena Gómez: Caracterización química de tornillos de PLGA para fijación de fracturas óseas. XXXVIII Congreso Nacional de Ingenieria Biomédica, Mazatlan, Sinaloa; 10/2015, DOI:10.1016/011313082200-02
Sasha Lizbeth Gomez Castellanos, Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Ana Leticia Iglesias, Eduardo Serena Gómez: Caracterización química de membranas de colágeno para la regeneración guiada de hueso. XXXVIII Congreso Nacional de Ingenieria Biomédica, Mazatlan, Sinaloa; 10/2015, DOI:10.1016/011313082200-01
Benjamin Gonzalez Vizcarra, Ana Gabriela Carrillo Varguez, Luis Jesús Villarreal Gómez, Fernando Palacios Rojas, Miguel Angel Avila Puc, Alberto Delgado Hernandez: Fabricación de un punzón para la realización de ensayos para evaluar la fuerza de empuje en selladores dentales. XXI CONGRESO INTERNACIONAL ANUAL DE LA SOCIEDAD MEXICANA DE INGENIERÍA MECÁNICA, Coatzacoalcos, Veracruz, México; 09/2015, DOI:10.1016/091414110400-203
Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Sasha Lizbeth Gómez Castellanos, Ana Leticia Iglesias, Eduardo Serena Gómez: CHEMICAL CHARACTERIZATION OF A SYNTHETIC COLLAGEN MEMBRANE FOR GUIDED BONE REGENERATION. XXIV International Materials Research Congress, Cancún, México; 08/2015, DOI:10.13140/RG.2.1.3036.8403
Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Sasha Lizbeth Gómez Castellanos, Ana Leticia Iglesias, Eduardo Serena Gómez: CHEMICAL CHARACTERIZATION OF A RESORBABLE SCREW FOR BONE FIXATION. XXIV International Materials Research Congress, Cancún, México; 08/2015, DOI:10.13140/RG.2.1.1463.9761
Ana Leticia Iglesias, Gerardo Quiñonez Garcia, Christian Romero Soto, Luis Jesús Villarreal Gómez: Síntesis y Caracterización de nuevos ligantes tipo Base de Schiff bis(imino)piridina y bis(imino)benceno derivadas de 3-amino-pirazol y sus complejos de coordinación con Cu(II).. 49 Congreso Mexicano de Química, Merida, Yucatán, México; 09/2014, DOI:10.13140/RG.2.1.5133.9925
Ricardo Vera−Graziano, Federico J. Sabina−Ciscar, Alfredo Maciel−Cerda, Francisco Sánchez- Arevalo, Raúl Montiel-Campos, Nikola Batina, José M. Cornejo-Bravo, Atlantida Raya-Rivera, Filiberto Rivera Torres, Edwin Y Gómez−Pachón, Alida Ospina-Orejarena, Luis J. Villarreal-Gómez, Selene Alcántara-Barrera, Lizeth Ávila Gutiérrez, Rodolfo J. Romero-Aragón, Andrómeda AL Monrroy Brera: SCAFFOLDS FOR BIOMEDICAL APPLICATIONS (MEMBRANAS P ARA USOS BIOMÉDICOS). IV Congreso Nacional de Membranas: Ciencia, Tecnología y Aplicaciones, Merida, Yucatan; 06/2014, DOI:10.1016/100211565900
David Abdel Mejia Medina, Luis Jesús Villarreal Gómez, Ana Leticia Iglesias, Victor H. Castillo: Análisis de Requerimientos para el Diseño de Herramientas en Soporte al Aprendizaje Integral de la Química. 9a Conferencia Ibérica de Sistemas y Tecnologías de Información, Barcelona, España; 06/2014, DOI:10.13140/RG.2.1.1037.9926
Ricardo Vera-Graziano, Federico J. Sabina-Ciscar, Alfredo Maciel-Cerda, Francisco Sánchez Arevalo, Raúl Montiel-Campos, Nikola Batina, José M. Cornejo-Bravo, Atlantida Raya- Rivera, Edwin Y. Gómez-Pachón, Alida Ospina-Orejarena, Luis J. Villarreal- Gómez, Selene Alcántara-Barrera, Lizeth Ávila Gutiérrez, Israel Morales- Reyes: Polymeric nanofiber scaffolds; Characterization and Modeling. XXVI Congreso Nacional De La Sociedad Polimérica De México, Coatzacoalcos, Veracruz, México; 11/2013, DOI:10.13140/RG.2.1.3135.1446
Luis Jesús Villarreal Gómez, Ricardo Vera Graziano, Maria Raquel Vega Rios, Jose Luis Pineda Camacho, Horacio Almanza Reyes, Jose Manuel Cornejo Bravo: EVALUACIÓN DE LA BIOCOMPATIBILIDAD DE ANDAMIOS DE POLI (L-LACTIDA) CON HIDROXIAPATITA PURA E INJERTADA PARA LA REGENERACIÓN DE TEJIDO DURO.. IV CONGRESO NACIONAL DE CIENCIA E INGENIERÍA EN MATERIALES, Pachuca, Hidalgo, México; 02/2013, DOI:10.13140/RG.2.1.2086.5682
Luis Jesús Villarreal Gómez, Irma Esthela Soria Mercado, Graciela Guerra Rivas, Nahara E. Ayala Sanchez: Actividad anticancerígena y antibacterial de extractos de algas marinas y bacterias asociadas a su superficie. 1er Congreso Regional de Bioingeniería, Tijuana, Baja California; 04/2011, DOI:10.13140/RG.2.1.4183.7202
Erick Torres, Lupita Elizabeth Martínez Topete, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Juvenal Pantoja, Aracely Serrano-Medina, Ana leticia Iglesias, Graciela Lizeth Pérez González, Luis Jesús Villarreal-Gómez, Tanya Bustamante Reynoso: Fabricación de Membranas Poliméricas Cargadas con Citrato de Sildenafil como Sistema de Liberación de Fármaco para el posible tratamiento de Hipertensión Arterial Pulmonar Crónica. VII Congreso Nacional de la Sociedad Mexicana de Ciencia y Tecnología de Membranas, A.C., Guanajuato, Guanajuato, México; 06/2018
Luis Jesús Villarreal-Gómez, Erick Torres, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Juvenal Pantoja, Aracely Serrano-Medina, Ana leticia Iglesias, Graciela Lizeth Pérez González: Poly (Vinyl Pyrrolidone) Nanofibers as Drug Delivery of Sildenafil Citrate. 2nd Edition of Global Conference on Pharmaceutics and Drug Delivery Systems, Roma, Italia; 06/2018
Erick Torres, Graciela Lizeth Pérez González, Aracely Serrano Medina, Daniel Grande, Ricardo Vera-Graziano, José Manuel Cornejo-Bravo, Luis Jesús Villarreal-Gómez: Electrospun Microfibers Containing Sildenafil Citrate for Fast Dissolving Delivery, for Possible Treatment of Chronic Pulmonary Hypertension. IV Symposium of Nanosciences and Nanomaterials, Ensenada, Baja California, México; 04/2018
Daniella Alejandra Pompa-Monroy, Ricardo Vera-Graziano, Syed Dastager, Karla Juarez-Moreno, N. Bogdanchikova, Irma Esthela Soria Mercado, Ana leticia Iglesias, Meghana Thorat, Luis Jesús Villarreal-Gómez: Gelatin/Collagen Scaffolds and Their Potential Use as a Bacterial Growth Promoter. IV Symposium of Nanoscience and Nanomaterials, Ensenada, Baja California, México; 04/2018
Paola Rachel Rodríguez Medrano, Karen Beldai Robles Campillo, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Hermelinda De la Cruz Durán, Ana leticia Iglesias, Aracely Serrano-Medina, Luis Jesús Villarreal-Gómez: Nanofibras de Poli (vinil pirrolidona) /Propanolol como Potencial Tratamiento en Hemangioma Infantil. 1er Congreso Internacional de Ciencias de la Ingeniería y Tecnología (ECITEC 2017), Tijuana, Baja California, México; 09/2017
Erick Torres, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, José Manuel Cervantes-Uc, López Maldonado Eduardo Alberto, Ana leticia Iglesias, Aracely Serrano Medina, Luis Jesús Villarreal-Gómez, Tanya Bustamante Reynoso: Estudio de Nanofibras de Poli (Vinil Pirrolidona) y Poli (Vinil Alcohol) Cargados con Sildenafil como Sistemas de Liberación de Fármaco. 1er Congreso Internacional de Ciencias de la Ingeniería y Tecnología (ECITEC 2017), Tijuana, Baja California, México; 09/2017
Paola Rachel Rodríguez Medrano, Luis Jesús Villarreal-Gómez, Karen Beldai Robles Campillo, José Manuel Cornejo-Bravo, Ricardo Vera-Graziano, Karla Oyuki Juárez Moreno, Hermelinda De la Cruz-Durán, Ana leticia Iglesias, Aracely Serrano-Medina: Poly (Vinyl Pirrolidone)/Propanolol Nanofibers as a Potential Treatment of Child Hemangioma. International Conference on Polymers and Advanced Materials POLYMAT-2017, Huatulco, Oaxaca, México; 09/2017
Alan Alvarez, Rodolfo Velasco, Vareska Lucero Zárate-Córdoba, Luis Jesús Villarreal-Gómez, Ana leticia Iglesias, E.A. López-Maldonado, Mercedes Oropeza: Producing Porous Scaffolds for Tissue Engineering: A comparative Analysis between Three Different Methods. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.20775.75689
Rodolfo Velasco, Alan Alvarez, Luis Jesús Villarreal-Gómez, Ana leticia Iglesias, E.A. López-Maldonado, Mercedes Oropeza: Evaluating the Bacterial Adsorption on Electrospun Nanofibers. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.21614.61762
Dayana Michelle Gómez Quintana, Luis Jesús Villarreal-Gómez, Paz Gon-zález Juan Antonio, López Maldonado Eduardo Alberto, Mercedes Oropeza, Cervantes Uc José Manuel, Ricardo Vera-Graziano, Ana leticia Iglesias: Fabricación de Nanofibras de PLGA con Recubrimiento de Polianilina para Aplicación en Ingeniería de Tisular. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.28325.50407
Juliane Jacqueline Coronel-Hurtado, Luis Jesús Villarreal-Gómez, Juan Antonio Paz-González, E.A. López-Maldonado, Teresita Merce-des Oropeza-Guzmán, José Manuel Cervantes-Uc, Ricardo Vera-Graziano, Ana leticia Iglesias: Fabrication of a Composite Material System as Potential Prosthetic Material. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.14903.73126
Vareska Lucero Zárate-Córdova, Luis Jesús Villarreal-Gómez, E.A. López-Maldonado, María Teresita Oropeza-Guzmán, Juan Antonio Paz-González, Ana leticia Iglesias: A Methodology for Fabrication of Hydroxyapatite and Fluoropatite Porous Scaffolds by Salt Leaching Technique for Bone Tissue Regeneration. XXV International Materials Research Congress 2016, Cancún, Quintana Roo, México; 08/2016, DOI:10.13140/RG.2.2.35036.39043
Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Jose Trinidad Lopez Maldonado, Luis Jesús Villarreal Gómez: Biopolímeros naturales y modificados con aplicaciones en medio ambiente, salud y agricultura sustentable. Congreso de Investigación Academia de Journals Tabasco 2016, Villahermosa, Tabasco; 03/2016
Vareska Zarate Cordova, Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Benjamin Gonzalez Vizcarra, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez: SYNTHESIS OF BONE-REPLACEMENT MATERIAL BY SALT-LEACHING METHOD FOR IMPROVE BONE TISSUE REGENERATION. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.2512.5525
Rodolfo Daniel Velasco Barraza, Alan Saul Alvarez Suarez, Eduardo Alberto Lopez Maldonado, Mercedes Teresita Oropeza Guzman, Adriana Alvarez Andrade, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez: Fabricación y caracterización de nanofibras de diferentes polímeros por electrohilado como soporte de sustrato para la construcción de un futuro biosensor. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.4609.7047
Antonio Juarez Duran, Ana Leticia Iglesias, Valentin Miranda soto, Luis Jesús Villarreal Gómez: Síntesis de ligantes del tipo Bis(imino) bi-dentados y tri-dentados con anilina pentafluorada y 3-amino, 5-metil pirazol. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.1988.2649
Alan Saul alvarez Suarez, Eduardo alberto Lopez Maldonado, Maria Teresita Oropeza Guzman, Ana Leticia Iglesias, Luis Jesús Villarreal Gómez, Rodolfo Daniel Velasco Barraza: SÍNTESIS Y CARACTERIZACIÓN DE MATERIALES COMPUESTOS BIOABSORBIBLES PARA APLICACIONES OSEAS. X Simposio Internacional de Investigación en Química en la Frontera, Tijuana, Baja California; 11/2015, DOI:10.13140/RG.2.1.4085.4169
Sasha Lizbeth Gomez Castellanos, Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Ana Leticia Iglesias, Eduardo Serena Gómez: Caracterización química de tornillos de PLGA para fijación de fracturas óseas. XXXVIII Congreso Nacional de Ingenieria Biomédica, Mazatlan, Sinaloa; 10/2015, DOI:10.1016/011313082200-02
Sasha Lizbeth Gomez Castellanos, Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Ana Leticia Iglesias, Eduardo Serena Gómez: Caracterización química de membranas de colágeno para la regeneración guiada de hueso. XXXVIII Congreso Nacional de Ingenieria Biomédica, Mazatlan, Sinaloa; 10/2015, DOI:10.1016/011313082200-01
Benjamin Gonzalez Vizcarra, Ana Gabriela Carrillo Varguez, Luis Jesús Villarreal Gómez, Fernando Palacios Rojas, Miguel Angel Avila Puc, Alberto Delgado Hernandez: Fabricación de un punzón para la realización de ensayos para evaluar la fuerza de empuje en selladores dentales. XXI CONGRESO INTERNACIONAL ANUAL DE LA SOCIEDAD MEXICANA DE INGENIERÍA MECÁNICA, Coatzacoalcos, Veracruz, México; 09/2015, DOI:10.1016/091414110400-203
Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Sasha Lizbeth Gómez Castellanos, Ana Leticia Iglesias, Eduardo Serena Gómez: CHEMICAL CHARACTERIZATION OF A SYNTHETIC COLLAGEN MEMBRANE FOR GUIDED BONE REGENERATION. XXIV International Materials Research Congress, Cancún, México; 08/2015, DOI:10.13140/RG.2.1.3036.8403
Luis Jesús Villarreal Gómez, Theodore Ng, Ricardo Vera Graziano, Sasha Lizbeth Gómez Castellanos, Ana Leticia Iglesias, Eduardo Serena Gómez: CHEMICAL CHARACTERIZATION OF A RESORBABLE SCREW FOR BONE FIXATION. XXIV International Materials Research Congress, Cancún, México; 08/2015, DOI:10.13140/RG.2.1.1463.9761
Ana Leticia Iglesias, Gerardo Quiñonez Garcia, Christian Romero Soto, Luis Jesús Villarreal Gómez: Síntesis y Caracterización de nuevos ligantes tipo Base de Schiff bis(imino)piridina y bis(imino)benceno derivadas de 3-amino-pirazol y sus complejos de coordinación con Cu(II).. 49 Congreso Mexicano de Química, Merida, Yucatán, México; 09/2014, DOI:10.13140/RG.2.1.5133.9925
Ricardo Vera−Graziano, Federico J. Sabina−Ciscar, Alfredo Maciel−Cerda, Francisco Sánchez- Arevalo, Raúl Montiel-Campos, Nikola Batina, José M. Cornejo-Bravo, Atlantida Raya-Rivera, Filiberto Rivera Torres, Edwin Y Gómez−Pachón, Alida Ospina-Orejarena, Luis J. Villarreal-Gómez, Selene Alcántara-Barrera, Lizeth Ávila Gutiérrez, Rodolfo J. Romero-Aragón, Andrómeda AL Monrroy Brera: SCAFFOLDS FOR BIOMEDICAL APPLICATIONS (MEMBRANAS P ARA USOS BIOMÉDICOS). IV Congreso Nacional de Membranas: Ciencia, Tecnología y Aplicaciones, Merida, Yucatan; 06/2014, DOI:10.1016/100211565900
David Abdel Mejia Medina, Luis Jesús Villarreal Gómez, Ana Leticia Iglesias, Victor H. Castillo: Análisis de Requerimientos para el Diseño de Herramientas en Soporte al Aprendizaje Integral de la Química. 9a Conferencia Ibérica de Sistemas y Tecnologías de Información, Barcelona, España; 06/2014, DOI:10.13140/RG.2.1.1037.9926
Ricardo Vera-Graziano, Federico J. Sabina-Ciscar, Alfredo Maciel-Cerda, Francisco Sánchez Arevalo, Raúl Montiel-Campos, Nikola Batina, José M. Cornejo-Bravo, Atlantida Raya- Rivera, Edwin Y. Gómez-Pachón, Alida Ospina-Orejarena, Luis J. Villarreal- Gómez, Selene Alcántara-Barrera, Lizeth Ávila Gutiérrez, Israel Morales- Reyes: Polymeric nanofiber scaffolds; Characterization and Modeling. XXVI Congreso Nacional De La Sociedad Polimérica De México, Coatzacoalcos, Veracruz, México; 11/2013, DOI:10.13140/RG.2.1.3135.1446
Luis Jesús Villarreal Gómez, Ricardo Vera Graziano, Maria Raquel Vega Rios, Jose Luis Pineda Camacho, Horacio Almanza Reyes, Jose Manuel Cornejo Bravo: EVALUACIÓN DE LA BIOCOMPATIBILIDAD DE ANDAMIOS DE POLI (L-LACTIDA) CON HIDROXIAPATITA PURA E INJERTADA PARA LA REGENERACIÓN DE TEJIDO DURO.. IV CONGRESO NACIONAL DE CIENCIA E INGENIERÍA EN MATERIALES, Pachuca, Hidalgo, México; 02/2013, DOI:10.13140/RG.2.1.2086.5682
Luis Jesús Villarreal Gómez, Irma Esthela Soria Mercado, Graciela Guerra Rivas, Nahara E. Ayala Sanchez: Actividad anticancerígena y antibacterial de extractos de algas marinas y bacterias asociadas a su superficie. 1er Congreso Regional de Bioingeniería, Tijuana, Baja California; 04/2011, DOI:10.13140/RG.2.1.4183.7202
